IMM1436H - Techniques in Immunology

Priority in IMM1436H is given to students in the Applied Immunology MSc program.

Course Description

The course aims to build knowledge in common analytical techniques used in immunology: immunohistology and flow cytometry. It will teach how to stain tissue samples using immunofluorescence and immunohistochemistry, as well analyze single cell suspensions via flow cytometry. Additionally, methods in proper image/data quantification/analysis will be taught. The course will also cover the various applications of these techniques and will include lectures, tutorials and practicum sessions.

Using lectures, tutorials and practicum laboratory sessions the course will cover:

Immunohistology - historical uses of immunohistochemistry, how it complements other techniques, tissue preparation, fixation and processing and staining techniques. These practical topics will be combined with theory on the architecture of different immune tissues (spleen, lymph node, small intestine), as well as inflamed/diseased tissues (kidneys, spinal cord).

Flow cytometry - state-of-the-art innovations in cytometry; advanced modeling methods of acquired data; and applications in cell biology, biotechnology, immunology, cell signaling, fluorescent protein analysis, bead-based assays and cell sorting.

Getting familiar with imaging and Flow cytometry analysis -  We will use Image J to learn basic immunohistology analysis and Flowjo, LegendPlex software for Flow Cytometry analysis.

Ancillary Fees:

To help with the costs of running IMM1436H, an ancillary fee of $25 will be charged to students enrolled in the course.